Role of phosphate, calcium species and hydrogen peroxide on albumin protein adsorption on surface oxide of Ti6Al4V alloy

Abstract Protein adsorption and its conformational arrangements on the surface of metallic biomaterials directly influence biocompatibility degradation process during implant lifetime. However, presence various species such as phosphates, calcium hydrogen peroxide (H2O2) in human body not only control electrochemical interactions biomaterial but could also modify protein impact metal degradation. To this aim bovine serum albumin (BSA) adsorption, morphology, potential corrosion resistance a Ti6Al4V alloy was investigated different solutions, including sodium chloride (NaCl), phosphate-buffered saline (PBS) Hank's physiological solutions. The results indicated that PBS solution more resistant to than Hanks’ or NaCl Mott–Schottky analysis demonstrated all solutions containing BSA H2O2 had highest donor charge carrier. Scanning electron microscopy (SEM) images by changing from then Hanks’, morphology adsorbed changed globular unfolded shape large micronetwork fine micro-nanonetwork, accompanied gradual increase potential. Moreover, it figured out protein/substrate interface top were susceptible initiation owing potentials thus are preferable sites for corrosive counterions, e.g., Cl?.